Physiobiochemical and microbiologic stability characteristics of freeze-dried cartilage secretome Adipose Mesenchymal Stem Cell (AdMSC)

Cartilage is an avascular, alymphatic, and aneurysmal structure, so it difficult to heal properly if injury occurs. Using Chondrogenic Adipose Mesenchymal Stem Cell (AdMSC) secretome as adjuvant one of the therapeutic options overcome problem cartilage. Storage distribution problems must be solved we use this method. This study aims characterize Freeze-dried chondrogenic AdMSC secretome. Secretome's physical, biochemistry, microbiology stability are derived from study’s design prospective observational analytics research. cartilage was analyzed for physical (organoleptic changes (shape, color, odor), pH, biochemistry (TGF-β3), (microbe contamination) stability. The difference observed in 0, 4, 8, 12 weeks. Data normality homogeneity were tested using Shapiro-Wilk test followed up with Friedman Wilcoxon tests analyze data further. Organoleptics change showed no color until four weeks shape smell No significant found porosity pH change—TGF-β3, which has a role chondrogenesis, considerable decrease contamination